Journal: Biomedicines
Article Title: Immunogenicity of RSV Fusion Protein Adsorbed to Non-Pathogenic Bacillus subtilis Spores: Implications for Mucosal Vaccine Delivery in Nonclinical Animal Models
doi: 10.3390/biomedicines13051112
Figure Lengend Snippet: Cotton rats were all immunized intranasally using strict intranasal or intramuscular immunization methods while alert at a volume of 0.1 mL (0.05 mL per leg). Groups were immunized three times except as indicated below. Strict intranasal immunization consisted of 0.01 mL volume delivered to the nostrils of each animal anesthetized with ketamine (40–90 µg per kg) and xylazine (5–10 mg per kg). The groups consisted of no vaccine; live RSV A2 virus; 0.2 mcg F protein-equivalent inactivated RSV with or without iSpores; 2 mcg Pre F + iSpores administered one, two, or three times; and Pre F with live spores or aluminum. In addition, IM groups using 2 mcg of Pre F with iSpores or aluminum were included. Seven weeks after the initial immunization, animals under isoflurane anesthesia were infected with RSV A2. Lung and nose tissues were obtained four days post-infection.
Article Snippet: Virus stocks Respiratory syncytial virus (RSV) strain A2 (ATCC VR-1540) and Long (ATCCVR-26 TM) were generated by infecting HEp-2 cells (ATCC, CCL-23).
Techniques: Virus, Infection