rsv virus stock  (ATCC)

Journal: medRxiv

Article Title: Development and Evaluation of an ARTIC-Based Amplicon Sequencing Assay for Whole-Genome Characterization of Respiratory Syncytial Virus

doi: 10.64898/2026.04.06.26350258

Figure Lengend Snippet: A custom, in-house bioinformatics pipeline was used to detect, subtype, and generate high-quality consensus sequences for RSV-A and RSV-B.

Article Snippet: Intact synthetic RNA transcripts of RSV-A and RSV-B (ZeptoMetrix: RSV-A Cat. No. NATRSVA-STQ, RSV-B Cat. No. NATFRC-ERC; ATCC: RSV-A Cat. No. VR-3418, RSV-B Cat. No. VR-1400) were run in duplicate as positive controls, while nuclease-free water served as a negative extraction control.

Techniques:

Journal: medRxiv

Article Title: Development and Evaluation of an ARTIC-Based Amplicon Sequencing Assay for Whole-Genome Characterization of Respiratory Syncytial Virus

doi: 10.64898/2026.04.06.26350258

Figure Lengend Snippet: A) Genomic coverage and B) mean depth of coverage plots of high-quality RSV-A (75) samples and RSV-B samples (76). The boxplot values indicate the overall median percent coverage and depth: 98% and 53,434x for RSV-A, and 98% and 48,585x for RSV-B. C) Genomic coverage map for RSV-A; (Genomic coordinates: NS1 70-489, NS2 599-973, N 1111-2286, P 2318-3043, M 3226-3996, SH 4266-4460, G 4652-5617, F 5697-7421, M2-1 7640-8224, M2-2 8199-8465, L 8532-15029). D) Genomic coverage map for RSV-B; (Genomic coordinates: NS1 57-475, NS2 584-958, N 1097-2272, P 2305-3030, M 3154-3990, SH 4259-4456, G 4645-5578, F 5676-7400, M2-1 7627-8214, M2-2 8180-8452, L 8518-15018), showing all passing samples and primer pair positions. The dotted line indicates the median depth of coverage.

Article Snippet: Intact synthetic RNA transcripts of RSV-A and RSV-B (ZeptoMetrix: RSV-A Cat. No. NATRSVA-STQ, RSV-B Cat. No. NATFRC-ERC; ATCC: RSV-A Cat. No. VR-3418, RSV-B Cat. No. VR-1400) were run in duplicate as positive controls, while nuclease-free water served as a negative extraction control.

Techniques:

Journal: medRxiv

Article Title: Development and Evaluation of an ARTIC-Based Amplicon Sequencing Assay for Whole-Genome Characterization of Respiratory Syncytial Virus

doi: 10.64898/2026.04.06.26350258

Figure Lengend Snippet: Probit regression analysis estimating the lower limit of detection as 4.4 TCID 50 /mL for (A) RSV-A and 18.6 TCID 50 /mL for (B) RSV-B. Data points at the top of each panel represent infinitive values.

Article Snippet: Intact synthetic RNA transcripts of RSV-A and RSV-B (ZeptoMetrix: RSV-A Cat. No. NATRSVA-STQ, RSV-B Cat. No. NATFRC-ERC; ATCC: RSV-A Cat. No. VR-3418, RSV-B Cat. No. VR-1400) were run in duplicate as positive controls, while nuclease-free water served as a negative extraction control.

Techniques:

Journal: medRxiv

Article Title: Development and Evaluation of an ARTIC-Based Amplicon Sequencing Assay for Whole-Genome Characterization of Respiratory Syncytial Virus

doi: 10.64898/2026.04.06.26350258

Figure Lengend Snippet: B) Pie charts show the distribution of clades in the sample sets. For RSV-A, the dominant clades were A.D.3.1 (n = 26) and A.D.5.2 (n = 21), together comprising 63% (47/75) of the passing samples. For RSV-B, the dominant clade is B.D.E.1 (n = 69), representing 91% (69/76) of passing samples. The maximum likelihood phylogenetic trees of (C) RSV-A and (D) RSV-B genomes generated in Nextclade.

Article Snippet: Intact synthetic RNA transcripts of RSV-A and RSV-B (ZeptoMetrix: RSV-A Cat. No. NATRSVA-STQ, RSV-B Cat. No. NATFRC-ERC; ATCC: RSV-A Cat. No. VR-3418, RSV-B Cat. No. VR-1400) were run in duplicate as positive controls, while nuclease-free water served as a negative extraction control.

Techniques: Generated

Journal: Biomedicines

Article Title: Immunogenicity of RSV Fusion Protein Adsorbed to Non-Pathogenic Bacillus subtilis Spores: Implications for Mucosal Vaccine Delivery in Nonclinical Animal Models

doi: 10.3390/biomedicines13051112

Figure Lengend Snippet: Immune Responses and Viral Load in Cotton Rats Following Strict Intranasal Immunization. Just prior to infection, serum was collected from each animal for antibody determinations. ( A ) Binding antibody was determined via ELISA. ( B ) Serum-neutralizing titers (NT50) were determined from the same blood sample. Four days after infection of the animals with RSV A2, ( C ) lung and ( D ) nose homogenates were evaluated for viral load in each sample type.

Article Snippet: Virus stocks Respiratory syncytial virus (RSV) strain A2 (ATCC VR-1540) and Long (ATCCVR-26 TM) were generated by infecting HEp-2 cells (ATCC, CCL-23).

Techniques: Infection, Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Biomedicines

Article Title: Immunogenicity of RSV Fusion Protein Adsorbed to Non-Pathogenic Bacillus subtilis Spores: Implications for Mucosal Vaccine Delivery in Nonclinical Animal Models

doi: 10.3390/biomedicines13051112

Figure Lengend Snippet: Cotton rats were all immunized intranasally using strict intranasal or intramuscular immunization methods while alert at a volume of 0.1 mL (0.05 mL per leg). Groups were immunized three times except as indicated below. Strict intranasal immunization consisted of 0.01 mL volume delivered to the nostrils of each animal anesthetized with ketamine (40–90 µg per kg) and xylazine (5–10 mg per kg). The groups consisted of no vaccine; live RSV A2 virus; 0.2 mcg F protein-equivalent inactivated RSV with or without iSpores; 2 mcg Pre F + iSpores administered one, two, or three times; and Pre F with live spores or aluminum. In addition, IM groups using 2 mcg of Pre F with iSpores or aluminum were included. Seven weeks after the initial immunization, animals under isoflurane anesthesia were infected with RSV A2. Lung and nose tissues were obtained four days post-infection.

Article Snippet: Virus stocks Respiratory syncytial virus (RSV) strain A2 (ATCC VR-1540) and Long (ATCCVR-26 TM) were generated by infecting HEp-2 cells (ATCC, CCL-23).

Techniques: Virus, Infection